Life Length’s Telomere Analysis Technologies are the most valuable, versatile and scalable technologies in the market. While some companies offer mean telomere length analyses, Life Length is the only company in the world able to offer a highly scalable technology to measure short telomeres in individual cells from both blood and tissue samples, which is the relevant indicator of telomere dysfunction and cellular aging.
TAT® is a proprietary technology that assesses telomere length individually. We measure telomere length using high-content screening, high throughput in situ fluorescence hybridization, providing a comprehensive analysis of the entire distribution of telomere length ranging from the shortest to the longest telomeres, including frequency, mean and median values.
Q-TRAP measures telomerase activity in cellular extracts. We detect relative telomerase activity by a modified version of the telomeric repeat amplification protocol coupled with a real-time quantitative PCR analysis (Q-TRAP). The methodology has been optimized and scaled up to the highest standards offering a reliable, sensitive, and rapid determination that can be combined with our TAT® and other biomarkers.
Reproducibility & Scalability
Telomapping® is a technology that assesses semi-quantitatively telomere length in tissue samples. We detect relative telomere length by in situ fluorescence hybridization in tissue biopsies. This method retains the advantages of exquisite sensitivity, the depth of data derived from high content screening and the ability to compare, within the same tissue cut, control and test tissue – cancerous, treated, etc.
Tissue cuts from paraffin fixed biopsies. Thickness should be between 10μm-20μm
Ideally three tissue sections per slide. When comparing normal and test tissue include both on the same slide.
Pack slides, properly wrapped, and ship at room temperature.
TRF is a technology that assesses telomere length from purified DNA of biological samples. This technique reports absolute telomere length using southern blot analysis of fragmented genomic DNA. Average telomere length is calculated using reference DNA size markers. This technique allows telomere length determination when only DNA is available.
Quality Polymerase Chain Reaction can be performed in samples where only DNA is available…