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LifeLengthTech®

Telomere & Cell Biology Platform

LifeLengthTech® is our proprietary platform to generate high-precision, clinically relevant insights from telomeres and cellular aging biomarkers.
Discover how it works

Single-cell telomere insights

State of the art technology for early diagnostics

TAT® – Telomere Analysis Technology, the only FDA- and CE-IVD-approved technology capable of measuring telomere length at the level of individual chromosomes across millions of cells. Layered on top, we combine telomerase activity, ALT, high-content imaging and targeted gene expression to deliver decision-ready biomarkers for oncology, longevity & personalized medicine, and R&D/CRO programs.
Single-cell
Confocal Microscopy
High-throughput
Deliverables: standardized clinical-style reports (with reference ranges and color-coded zones), raw and processed datasets, and optional statistical analysis for trials.

Confocal Microscopy

High-content imaging for accurate information

Life Length runs a high-content confocal platform (HC Opera) that few labs have in routine use. Combined with our assays (HT-Q-FISH, RNAscope®, immunofluorescence), we can visualize and measure telomeres, DNA damage, senescence markers and subcellular localization across thousands of cells in a single run—turning stunning images into robust, decision-ready data under CLIA/ISO workflows.
See more in our technologies directory
Mitochondrial content
DNA damage (orange)
Cells & Chromosomes (ALT metaphase)
Actina
Oxidative stress & senescence in cells
Oxidative stress in cells
Cells (TAT®)
Cell senescence
Oxidative stress in cells

From sample to decisions

Standardized, reproducible and ready for action

Beyond telomeres, Life Length provides complementary biomarkers from non-invasive samples

Oxidative-stress balance, intracellular NAD+ status , mitochondrial content, cellular senescence and DNA damage among others.

Together they create multi-parametric profiles for research and clinical decision support.

Samples
Blood, cultured cells, tissue
Confocal acquisition

Automated imaging (384-well)

Assay panels
Telomeres, Senescence, ROS, Mito, γH2AX, RNA-ISH, qPCR telomerase, Inflammation
Analytics
Single-cell distributions & multi-parametric profiles
Decision support

CRO/CRS studies, oncology early diagnostics, longevity programs

What does it measure?

Telomeres & Telomerase

Single-cell TAT® analytics

Complete telomere profile (MTL, short-telomere P20) plus telomerase readouts (Q-TRAP activity, hTERT mRNA).

Cell State & DNA Damage

Senescence DSB markers
Single-cell SA-β-gal and γH2AX to detect senescence and double-strand breaks, with optional repair/protection assays.

Stress & Metabolism

Redox and mitochondria
Blood oxidative stress (ROM/UCARR), intracellular NAD+, and mitochondrial content/ΔΨm to track energetic and metabolic status.

Expression & Inflammation

RNA, protein endpoints
RNAscope® (RNA-ISH) for cell/tissue transcripts and cytokine panels by ELISA/EliSpot integrable with TAT® for targeted profiling.

LifeLengthTech® Directory

Find by application, sample type, technique or target

Filters

TAT® – HT Q-FISH

  • CRS, Longevity, Oncology
Measurements on approximately 10,000 cells and 300,000 individual telomeres. Extraction of > 220 TAVs (Telomere Associated Variables).
  • q-FISH (confocal/imaging)

Application

Sample type

Life Length patented Telomere Analysis Technology (TAT®) is based on a quantitative fluorescence in-situ hybridization (Q-FISH) method modified for cells in interphase.

In brief, telomeres are hybridized with a fluorescent Peptide Nucleic Acid (PNA) probe that recognizes three telomere repeats. The images of the nuclei and telomeres are captured by a high-content screen system. The intensities of fluorescence are translated to base pairs (bp) through a standard regression curve which is generated using control cell lines with known telomere length.

Data outputs:

  • Percentage of critically short telomeres (<3 Kbp)
  • Telomere length percentiles (0–99)
  • Median telomere length (MTL)

Robust protocol

  • Clinically validated (CLIA y ISO 15189)
  • 5 replicates per sample
  • Can be performed on any cell type or tissue
  • Simultaneous processing of hundreds of samples (384 plates)
  • Intra-assay coefficient of variation <5%
  • Seven control cell lines
  • Thousands of individual telomeres measured per sample
  • Full study report with statistical analysis included.

The TAT® technology distinguishes telomerase-positive from ALT (Alternative Lengthening of Telomeres) cells by identifying the bimodal telomere length distribution of very short and very long telomeres.

scripTERT (hTERT mRNA)

  • CRS
Robust quantitative RT-PCR assay for assessing the expression of hTERT.
  • qPCR

Application

Sample type

Using a custom TaqMan® real-time quantitative PCR assay to assess mRNA hTERT (the gene encoding the catalytic subunit of telomerase), this method enables sensitive and reproducible detection of telomerase gene activation.

Highlights of the assay:

  • Excellent assay linearity (standard curve R² > 0.98)
  • Direct quantification from whole cell lysates, minimizing sample preparation
  • Outstanding reproducibility with intra- and inter-assay variability below 0.4 Ct
  • Consistent and reliable results, with control samples maintained within ±1.2 Ct

RNAscope® (TAT®-ISH)

  • CRS
TAT®-ISH is an integrated assay combining RNAscope® RNA in situ hybridization with our Telomere Analysis Technology.
  • q-FISH (confocal/imaging), RNA-ISH

Application

Sample type

RNA expression detection in spatial context, in combination with telomere length analysis in specific subpopulations.

This allows telomere length measurement in phenotypically defined cells within mixed populations or tissues.

Q-TRAP

  • CRS
High sensitivity detection of telomerase-positive cells. Results expressed as Relative Telomerase Activity (RTA), normalized to control lines.
  • qPCR

Application

Sample type

Life Length offers an in-house qTRAP assay as a sensitive and quantitative alternative to traditional TRAP assays. This technology enables accurate measurement of telomerase activity across a wide range of sample types.

  • No need for gel electrophoresis
  • Quantitative and high-throughput
  • Validated with triplicates and statistical controls
  • Detection as low as 100 cells

Mitochondrial assays

  • CRS
Mitochondrial assays with three different approaches (Content, membrane potential & oxidative stress)
  • q-FISH (confocal/imaging)

Application

Sample type

Mitochondrial Content:
Staining with MitoTracker® Green FM to measure mitochondrial mass.

Mitochondrial Membrane Potential (MMP):
TMRM dye used to assess mitochondrial activity.

Mitochondrial ROS:
Staining with CellROX™ to measure mitochondrial oxidative stress.

Inflammation (ELISA)

  • CRS
We offer quantitative detection of pro- and anti-inflammatory cytokines in cell culture supernatants and blood samples.
  • ELISA, EliSpot, Fluorospot

Application

Sample type

Cytokines measured include:

IL-1β, IL-2, IL-6, IL-10, TNF-α (among others)

In vitro: LPS-induced macrophage inflammation model available. Evaluation of test compound efficacy in reducing inflammatory response.

In vivo: ELISA to detec specific cytokines

DNA Damage (γH2AX / UV-B)

  • CRS
DNA double-strand breaks by assessing γH2AX phosphorylation.
  • q-FISH (confocal/imaging)

Application

Sample type

Measures phosphorylation of H2AX at Ser139, detecting DNA double-strand breaks. Uses nuclear staining and quantification of γH2AX foci per nucleus after irradiating with UV-B light.

Cellular Senescence (SA-β-gal)

  • CRS
Identification of senescent cells based on the presence of increased lysosomal beta-galactosidase activity.
  • q-FISH (confocal/imaging)

Application

Sample type

Available for both non-induced (protective mechanism) and doxorubicin-induced (repairing mechanism) senescence .

Cellular ROS

  • CRS, Longevity & PM
CellROX™ fluorescence probe used to quantify oxidative stress in single cells or blood.
  • q-FISH (confocal/imaging)

Application

Sample type

Detection of intracellular reactive oxigen species (ROS)

ALT phenotype (via TAT®)

  • CRS
Detection of Alternative Lengthening of Telomeres (ALT) mechanism in tumor cell lines.
  • q-FISH (confocal/imaging)

Application

Sample type

The ALT mechanism is a telomerase-independent pathway in which tumor cells maintain telomere length through homologous recombination based in Life Length patented Telomere Analysis Technology (TAT®).

Life Length know-how in the market

Biomarkers for early oncology diagnostics and longevity & personalized medicine

ProsTAV®

Blood-based telomere biomarker that estimates clinically significant prostate cancer risk, helping reduce unnecessary biopsies and flag csPCa missed by MRI.
More info

PneumoTAV

Telomere-based biomarker combining multiple TAVs with ML to support earlier lung cancer detection and LDCT/nodule triage.
* Research use
Contact us for more info

HealthTAV®

Single-cell telomere analytics to quantify biological age (MTL & P20).
More info

HealthOX

Oxidative-stress level from blood (ROM/UCARR) reported by severity.
More info

HealthNAD+

Intracellular NAD+ to assess cellular energy and DNA-repair capacity.
More info

HealthG

Saliva-based qPCR genotyping, linked to appetite, metabolism and drug response.
More info

Got any questions?

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