Academic researchers in scientific investigation and studies | Life Length

Researchers

Life Length’s technologies are of higher quality, more accurate and provide faster results than any other company in the telomere field. We can also provide cell culture services for in vitro experiments and histology services for telomere analysis in tissue (Telomapping®).

 

By incorporating our telomere and telomerase assessments, many prestigious institutions, research centers and universities worldwide have been able to take their research projects to the next level by studying telomere biology with a granularity much higher than the one obtained by older techniques such as qPCR. Academic institutions and research centers can incorporate Life Length’s innovative telomere and telomerase assays in their studies easily and at attractive academic pricing.

OUR MEASUREMENTS CAN BE PERFORMED ON A WIDE VARIETY OF SAMPLE TYPES FOR ANY RESEARCH PROJECT

ISOLATED CELLS

ISOLATED CELLS

Determination of telomere length by chromosome from isolated cells by TAT®, based on proprietary high-throughput hybridization of specific probes using fluorescent microscopy in large format well plates

TISSUE-BIOPSIES

TISSUE-BIOPSIES

Determination of telomere length on chromosomes from tissue samples by hybridization of specific probes using Telomapping®

DNA

DNA

When DNA is available we determine telomere length using QualityPCR®

IN VITRO

IN VITRO

We help companies test and develop more efficient and healthier products in a wide range of industries such as pharma, consumer health and consumer products

IN VIVO

IN VIVO

We also perform telomere measurement in animals for experimental trials

CLINICAL

CLINICAL

Clinical analyses provided by Life Length help laboratories, medical companies and public institutions

Why should you measure telomeres with Life Length?

TELOMERE ANALYSIS TECHNOLOGY

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To measure median telomere length in any cell line, Life Length uses a high-throughput (HT) Q-FISH technique. This method is based on a quantitative fluorescence in situ hybridization method modified for cells in interphase1.

Example of telomere length distribution provided by TAT

It is telomere length distribution and especially the subset of short telomeres, rather than the mean telomere length, what triggers chromosome instability, senescence, and the aging process

TAT VALIDATION DATA

  • VALIDATION DATA shows a correlation of 0.99 with TRF.
  • VALIDATION DATA defines the limit of detection at 800 base pairs and demonstrates very high specificity.
  • VALIDATION DATA establish population curves -normal population data base from 18 to 85 years, to extrapolate patients’ data and generate reports.

TELOMERE ANALYSIS TECHNOLOGY VS OTHER TELOMERE LENGTH DETERMINATION TECHONOLOGIES

  • TAT reports the distribution of telomere length chromosome-by-chromosome on a per cell basis rather than just mean telomere length which older, less sensitive and granular techniques offer.

TAT sample requirements


1 Million cells per sample or 10 mL of freshly blood samples

Viability of cells greater than 60%

Minimum volumen of 300µl

Samples may be stored at -80ºC for a maximum of 2 months or in liquid nitrogen for long-term storage

Shipping to our laboratory is in dry-ice for stored samples

TELOMERE TECHNOLOGIES

TAT®

TAT®

Proprietary technology that quantifies telomere length individually (in blood samples and from cell culture)

TELOMAPPING®

TELOMAPPING®

Patented methodology telomere length is determined in tissue samples (biopsies)

TRAP

TRAP

Telomeric Repeat Amplification Protocol provides highly sensitive measurement of telomerase activity (in blood samples and from cell culture)

TRF

TRF

Terminal Repeat Fragmentation reports absolute telomere length using southern blot analysis of fragmented genomic DNA

Q-PCR

Q-PCR

Quality Polymerase Chain Reaction can be performed in samples where only DNA is available

TELOMERE STUDIES

In recent years the study of telomere biology and telomerase has been exponential. With the growing awareness of the crucial role that telomeres play in cellular health, major research centres worldwide have increasingly devoted efforts to the study of the mechanisms of cellular replication, senescence and the factors that impact on cell and viability and organismal aging. This is reflected in the number of scientific studies that have been published over the past 20 years which now exceed 20,000 peer-reviewed studies.

 

Telomere research has demonstrated to be particularly relevant in areas such as Cancer, stem cells and aging