Using a custom TaqMan® real-time quantitative PCR assay to assess mRNA hTERT (the gene encoding the catalytic subunit of telomerase), this method enables sensitive and reproducible detection of telomerase gene activation.
Highlights of the assay:
- Excellent assay linearity (standard curve R² > 0.98)
- Direct quantification from whole cell lysates, minimizing sample preparation
- Outstanding reproducibility with intra- and inter-assay variability below 0.4 Ct
- Consistent and reliable results, with control samples maintained within ±1.2 Ct